[HTML][HTML] Signaling molecules of the NF-κB pathway shuttle constitutively between cytoplasm and nucleus
A Birbach, P Gold, BR Binder, E Hofer… - Journal of Biological …, 2002 - ASBMB
We aimed to investigate the dynamics of the NF-κB signaling pathway in living cells using
GFP variants of p65-NF-κB, IκBα, tumor necrosis factor-receptor associated factor 2 (TRAF2),
the NF-κB inducing kinase (NIK) and IκB kinases (IKK1 and IKK2). Detailed kinetic analysis
of constitutive nucleocytoplasmic shuttling processes revealed that IκBα enters the nucleus
faster than p65. Examination of signaling molecules upstream of NF-κB and IκBα revealed a
predominant cytoplasmic localization at steady state. However, after addition of leptomycin …
GFP variants of p65-NF-κB, IκBα, tumor necrosis factor-receptor associated factor 2 (TRAF2),
the NF-κB inducing kinase (NIK) and IκB kinases (IKK1 and IKK2). Detailed kinetic analysis
of constitutive nucleocytoplasmic shuttling processes revealed that IκBα enters the nucleus
faster than p65. Examination of signaling molecules upstream of NF-κB and IκBα revealed a
predominant cytoplasmic localization at steady state. However, after addition of leptomycin …
