Hydroxyl radicals depolymerize glomerular heparan sulfate in vitro and in experimental nephrotic syndrome
CJI Raats, MAH Bakker, J van den Born… - Journal of Biological …, 1997 - ASBMB
Heparan sulfate, the polysaccharide side chain of heparan sulfate proteoglycan, is important
for the permselective properties of the glomerular basement membrane. In this report, we
show a role for hydroxyl radicals in heparan sulfate degradation and an enhanced
glomerular basement membrane permeability. First, in enzyme-linked immunosorbent
assay, exposure of coated heparan sulfate (proteoglycan) to reactive oxygen species
resulted in a±50% decrease of binding of a monoclonal antibody against heparan sulfate …
for the permselective properties of the glomerular basement membrane. In this report, we
show a role for hydroxyl radicals in heparan sulfate degradation and an enhanced
glomerular basement membrane permeability. First, in enzyme-linked immunosorbent
assay, exposure of coated heparan sulfate (proteoglycan) to reactive oxygen species
resulted in a±50% decrease of binding of a monoclonal antibody against heparan sulfate …
