ATR and H2AX cooperate in maintaining genome stability under replication stress
RA Chanoux, B Yin, KA Urtishak, A Asare… - Journal of biological …, 2009 - ASBMB
Chromosomal abnormalities are frequently caused by problems encountered during DNA
replication. Although the ATR-Chk1 pathway has previously been implicated in preventing
the collapse of stalled replication forks into double-strand breaks (DSB), the importance of
the response to fork collapse in ATR-deficient cells has not been well characterized. Herein,
we demonstrate that, upon stalled replication, ATR deficiency leads to the phosphorylation
of H2AX by ATM and DNA-PKcs and to the focal accumulation of Rad51, a marker of …
replication. Although the ATR-Chk1 pathway has previously been implicated in preventing
the collapse of stalled replication forks into double-strand breaks (DSB), the importance of
the response to fork collapse in ATR-deficient cells has not been well characterized. Herein,
we demonstrate that, upon stalled replication, ATR deficiency leads to the phosphorylation
of H2AX by ATM and DNA-PKcs and to the focal accumulation of Rad51, a marker of …
